|
KMID : 0858620070110010003
|
|
Korean Journal of Audiology
2007 Volume.11 No. 1 p.3 ~ p.8
|
|
|
Expression & Role of Neural Cell Adhesion Molecule and Polysialic Acid in Differentiation of Adult Neural Stem Cell Derived from Guinea Pig Spiral Ganglion
|
|
Park Kyoung-Ho
Shin Ji-Hyeon
Yun Chang-Hyun
Kim Jong-Hoon
Kim Nam-Sik
Cho Sang-Jae
Park Shi-Nae
Yeo Sang-Won
|
|
|
Abstract
|
|
|
Background and Objectives : In 2005, Helge Rask-Andersen and Park isolated and cultured adult neural stem cells specific to inner ear from Ginea pigs and human spiral ganglion successfully. The purpose of this study is to identify the expression of neural cell adhesion molecule from neural stem cells and to assess the influence of its glycosilation and deglycosilation to neural stem cell differentiation.
Materials and Methods : Spiral ganglions were isolated from anesthetized Ginea pigs. The cells were triturated by trypsinization and addition of DNase and cultured in the media including epidermal growth factor (EGF) and fibroblast growth factor (FGF). The cultured cells were stained with stem cell markers such as bromodeoxyuridine (BrdU) and nestin. These cells were stained with neural cell adhesion molecule (NCAM) and polysialic acid (polySia) to identify their expression. And endoneuraminidase (endo-N), the enzymes that remove polySia from NCAM, was added to the culture media for 24 hours and we checked the degree of the differentiation.
Results: The neural stem cells from spiral ganglion expressed NCAM and polySia. In the group with Endo-N, the differentiation of neural stem cells to neuron and glial cells were decreased.
Conclusion: Spiral ganglion neural stem cells express NCAM and polySia. The glycosilation of NCAM may have a important role in neural stem cell differentiation as a positive regulator.
|
|
|
KEYWORD
|
|
|
Neural stem cell, Neural cell adhesion molecule, Polysialic acid, Differentiation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|